Many technology business incubators(TBIs) in China are facing the pressure of business transformation. Actively exploring the innovative business model of TBIs will help TBIs to develop better and provide better services for innovation and entrepreneurship. In recent years, the role of integration of Government-Industry-University in innovation and entrepreneurship has been arising. Under the theory of the triple helix, using Analytic Network Process (ANP) to explore the role of innovation subjects of government, enterprises and universities will help TBIs develop better. The research shows that giving full play to the main role of the enterprise, the leading role of the government, and the support role of the university are of great significance for TBIs to achieve innovative management.