The paper explores the test on the internal innovational entrepreneurial environment of the company based on entrepreneurial environment, and filter the items after pre-survey according to the survey of five dimensions about innovational entrepreneurial environment, then collate and analyze the original data, and carry out the exploratory factors analysis and confirmatory factors analysis of the scale, and verify the testing on innovational entrepreneurial environment of the company from the perspective of reliability and validity. Finally, the paper studies the effectiveness of the internal innovational entrepreneurship environment, As a result, the data indicate the scale of innovational entrepreneurial environment of the company including management support，resource support，compensation support，organizational culture and organizational structure has good reliability and validity. The perception of internal innovational entrepreneurship environment in enterprises has a significant positive impact on the internal innovational entrepreneurship behavior of employees. Psychological empowerment completely mediates the influence of various dimensions of the perception of internal innovational entrepreneurship environment in enterprises on the internal innovational entrepreneurship behaviors of employees.