Deviant innovation is an innovative way based on employees'' active behavior, which is constrained by organizational system and management style. Managerial Pro-social Behaviour can create a broad and independent working atmosphere, which is conducive to knowledge workers to break through the shackles of time and resources, and finally achieve breakthrough organizational performance. Taking high-tech enterprises as examples, this paper constructs an analysis framework with managerial pro-social behaviour as independent variables, deviant innovation as dependent variables, regulatory focus as intermediary variables and work autonomy as regulatory variables, and uses Amos Structural Equation Model to explore the action path. The results show that managerial pro-social behaviour have a positive impact on deviant innovation, regulatory focus is used as a positive intermediary between managerial pro-social violations and deviant innovation, and work autonomy plays a regulatory role in the relationship between managerial pro-social behaviour and deviant innovation.