This paper analyzes the main body relationship and advantages in the ecosystem of high-tech industry, analyzes the evolution effect among the main body relationship in the ecosystem based on lotka-volterra model, and constructs the entropy change model to analyze the collaborative mechanism of the system and the external environment evolution. The results show that: 1. When the inter-subject relationship evolves into moderate competition, mutualism and favoritism, the equilibrium output of the system group and individual is relatively large; 2. When the relationship between subjects is parasitic symbiosis, the equilibrium output of the system population and individual is small. 2. When the marginal collaborative entropy is equal to the marginal collaborative negative entropy, the collaborative efficiency of the ecosystem is the highest; The condition for the sustainable and stable development of the system is that the subject learns dynamically and introduces negative entropy flow from the outside to restrain the increase of collaborative entropy inside the system. 3. Ability matching between subjects is an important prerequisite for improving system performance.